PBS缓冲液的配制,pbs缓冲液的配制ph7.4

('PBS缓冲液的配制PBS是最普遍不过的实验室缓冲液，但其配方各异。以下是总结：母液的制：0.2MNa2HPO4：称取71.6gNa2HPO4-12H2O，溶于1000ml水0.2MNaH2PO4：称取31.2gNaH2PO4-2H2O，溶于1000ml水各种浓度PB(pH=7.4)的配制：先配0.2MPB(pH=7.4，100ml)：取19ml0.2mol...PBS是最普遍不过的实验室缓冲液，但其配方各异。以下是总结：母液的配制：0.2MNa2HPO4：称取71.6gNa2HPO4-12H2O，溶于1000ml水0.2MNaH2PO4：称取31.2gNaH2PO4-2H2O，溶于1000ml水各种浓度PB(pH=7.4)的配制：先配0.2MPB(pH=7.4，100ml)：取19ml0.2mol/L的NaH2PO4，81ml0.2mol/L的Na2HPO4,即可。然后只需将0.2MPB(pH=7.4)按相应比例适当稀释即可，如：0.1MPB（PH=7.4）：取500ml0.2MPB，加水稀释至1000ml即可。0.01MPB（PH=7.4）：取50ml0.2MPB，加水稀释至1000ml即可。0.02MPB（PH=7.4）：取100ml0.2MPB，加水稀释至1000ml即可。若需要NaCl的话，加入NaCl至0.9%（g/100ml）即可。另：其它各种另PH值的0.2MPB（100ml)配方：pH0.2MNaH2PO4（ml）0.2MNa2HPO4（ml）5.793.56.55.89285.990106.087.712.36.185156.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851496.945557.038627.133677.228727.323777.419ml81ml7.516847.613877.710.50.57.88.591.57.97938.05.394.7Na2HPO4若为\uf082,NAH2PO4若为\uf081。则配制5LPBS计算方法如下：母液：0.1M\uf0821000ml取35.8g;0.1M\uf0811000ml取15.6g.0.1M100mlPH=7.5:取16ml\uf081+84ml\uf082；现配制5000ml0.1Mph=7.5PBS:则需要800ml\uf081+4200ml\uf082;将其换成克数800/100015.6g\uf081=12.48g（将\uf081溶于800ml水中即可）4200/100035.8g\uf082=150.36g（将\uf082溶于4200水中即可）两者加起来是5000ml.',)